Bioremediation of Oil-contaminated Sand

The energy demand is set to grow very rapidly and the potential demand for oil around the world is at its highest level. Apart from indigenous oil sources, crude oil is imported by water transportation to fulfill local demand. The occurrence of oil leakage during drilling and transportation in pipelines is a major concern. As a result of this, soil is getting polluted and its geotechnical properties are altered. In this study, the effect of engine oil and diesel contamination on the geotechnical parameters of sea sand has been studied. Further, to enhance the properties of oil-contaminated sand, the bioremediation method was adopted. Sea sand from Ganagalla Peta beach, Andhra Pradesh state, India was taken up for the study. In the laboratory, 4%, 8%, 12% and 16% of engine oil and diesel were used to artificially contaminate the sea sand and geotechnical parameters; namely, compaction, shear strength and permeability, were studied for oil-contaminated sand and compared with those of virgin sea sand. In the next stage, bioremediation of engine oil-and diesel-contaminated sand was carried out to improve the geotechnical properties. It has been found that the geotechnical properties had improved after three days of bioremediation. Fourier transform infrared spectroscopy (FTIR) analysis showed that the contaminated sand is of a lipopeptide nature and showed the presence of carboxyl groups, whereas the IR absorption pattern of the treated sand matches with N-Methyl-N-Vinyl Acetamide. KEYWORDS: Engine-oil contamination, Diesel contamination, Bioremediation, Bacillus subtilis, Geotechnical properties, Compaction characteristics, Shear-strength characteristics, Permeability, FTIR analysis.