Study on Screening Microorganisms with Resistance to Parathion-Methyl and Degradation Characteristics

Toxicity of organophosphorus pesticide and the use of non-standard, which was the main factor of soil pesticides pollution, directly or indirectly harm human health. This study screened three wild species of bacteria, from the soil contaminated by pesticides in long-term, and respectively name as A1, A2 and A3. According to the bacterial 16S rDNA gene sequence analysis, combining with bacterial morphology, physiological and biochemical experiment results, and referring to "Berger bacterial identification manuals (eighth edition)", identified the three strains as Bacillus Branch, Clostridium microorganisms. A1, A2 and A3 were respectively Bacillus pasteurii, Clostridium butyricum and Clostridium acetobutylicum. Through the strain’s degradation and tolerability experiment to parathion-methyl, it turned out that the three strains reached the highest degradation rate in 12h, which was 73.04%, 78.05% and 85.51%, respectively.